yocK [2018-02-11T09:26:30.000Z]

general stress protein

locus: BSU19240

pI: 4.70

mw: 24.00 kDa

protein length: 209 aa

gene length: 627 bp

function: survival of ethanol stress

product: general stress protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

Gene

Coordinates: 2,097,126 → 2,097,617

The protein

Protein family

• azoreductase type 1 family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|8AB50581EBB3C27BB9A96EA3382F8C90CE5B6EC5|YlyA], [protein|70A5A147B908001878161D45AAEB08132AD0DB8C|YteA]

Expression and Regulation

Operons

• genes: [gene|633F6F54AFA144D4F65EA36B8D5670D3FC25EDA6|yocK]
  description: [Pubmed|10220166]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|10220166,15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|10220166,15805528]

Biological materials

Mutant

• MGNA-A322 (yocK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/322 NBRP B. subtilis, Japan]
• BKE19240 (Δ[gene|633F6F54AFA144D4F65EA36B8D5670D3FC25EDA6|yocK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTATATCCTCTCCTTTC, downstream forward: _UP4_TAAAAATGCCAAGCAAGTAC
• BKK19240 (Δ[gene|633F6F54AFA144D4F65EA36B8D5670D3FC25EDA6|yocK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTATATCCTCTCCTTTC, downstream forward: _UP4_TAAAAATGCCAAGCAAGTAC

References

• 10220166,15805528